Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Filtered Search Results
MilliporeSigma™ Calbiochem™ OmniPur™ 10X TAE Buffer, Liquid Concentrate
For use in molecular biology applications
EUROIMMUN Salt for PBS Buffer
These materials, used to create a PBS buffer for use on some Dynex platforms, help to control assay drift, and work to provide the best sensitivity and specificity in results
PIPES, 0.5M buffer soln., pH 5.5, Thermo Scientific™
CAS: 5625-37-6 Molecular Formula: C8H18N2O6S2 Molecular Weight (g/mol): 302.36 MDL Number: MFCD00006159 InChI Key: IHPYMWDTONKSCO-UHFFFAOYSA-N PubChem CID: 79723 ChEBI: CHEBI:44933 IUPAC Name: 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
| PubChem CID | 79723 |
|---|---|
| CAS | 5625-37-6 |
| Molecular Weight (g/mol) | 302.36 |
| ChEBI | CHEBI:44933 |
| MDL Number | MFCD00006159 |
| SMILES | C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O |
| IUPAC Name | 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid |
| InChI Key | IHPYMWDTONKSCO-UHFFFAOYSA-N |
| Molecular Formula | C8H18N2O6S2 |
CHES, 0.5M buffer soln, pH 10.0, Thermo Scientific™
CAS: 103-47-9 Molecular Formula: C8H17NO3S Molecular Weight (g/mol): 207.288 MDL Number: MFCD00003835 InChI Key: MKWKNSIESPFAQN-UHFFFAOYSA-N PubChem CID: 66898 IUPAC Name: 2-(cyclohexylamino)ethanesulfonic acid SMILES: C1CCC(CC1)NCCS(=O)(=O)O
| PubChem CID | 66898 |
|---|---|
| CAS | 103-47-9 |
| Molecular Weight (g/mol) | 207.288 |
| MDL Number | MFCD00003835 |
| SMILES | C1CCC(CC1)NCCS(=O)(=O)O |
| IUPAC Name | 2-(cyclohexylamino)ethanesulfonic acid |
| InChI Key | MKWKNSIESPFAQN-UHFFFAOYSA-N |
| Molecular Formula | C8H17NO3S |
| CAS | 102029-60-7 |
|---|---|
| Molecular Weight (g/mol) | 249.257 |
| Color | Colorless |
| Physical Form | Liquid |
| pH | 8.5 |
| Recommended Storage | Keep cold |
| Concentration | 0.2 M |
| Odor | Odorless |
MES, 0.2M buffer soln., pH 7.4, low endotoxin, Thermo Scientific™
CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.248 MDL Number: MFCD00283848 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N PubChem CID: 16218417 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid;hydrate SMILES: C1COCCN1CCS(=O)(=O)O.O
| PubChem CID | 16218417 |
|---|---|
| CAS | 145224-94-8 |
| Molecular Weight (g/mol) | 213.248 |
| MDL Number | MFCD00283848 |
| SMILES | C1COCCN1CCS(=O)(=O)O.O |
| IUPAC Name | 2-morpholin-4-ylethanesulfonic acid;hydrate |
| InChI Key | MIIIXQJBDGSIKL-UHFFFAOYSA-N |
| Molecular Formula | C6H15NO5S |
PIPES, 0.2M buffer soln., pH 6.8, low endotoxin, Thermo Scientific™
CAS: 5625-37-6 Molecular Formula: C8H18N2O6S2 Molecular Weight (g/mol): 302.36 MDL Number: MFCD00006159 InChI Key: IHPYMWDTONKSCO-UHFFFAOYSA-N PubChem CID: 79723 ChEBI: CHEBI:44933 IUPAC Name: 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
| PubChem CID | 79723 |
|---|---|
| CAS | 5625-37-6 |
| Molecular Weight (g/mol) | 302.36 |
| ChEBI | CHEBI:44933 |
| MDL Number | MFCD00006159 |
| SMILES | C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O |
| IUPAC Name | 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid |
| InChI Key | IHPYMWDTONKSCO-UHFFFAOYSA-N |
| Molecular Formula | C8H18N2O6S2 |
Bicine, 0.2M buffer soln., pH 9.0, low endotoxin, Thermo Scientific™
CAS: 150-25-4 Molecular Formula: C6H12NNaO4 Molecular Weight (g/mol): 185.16 MDL Number: MFCD00004295 InChI Key: MFBDBXAVPLFMNJ-UHFFFAOYSA-M PubChem CID: 8761 ChEBI: CHEBI:40957 IUPAC Name: sodium 2-[bis(2-hydroxyethyl)amino]acetate SMILES: [Na+].OCCN(CCO)CC([O-])=O
| PubChem CID | 8761 |
|---|---|
| CAS | 150-25-4 |
| Molecular Weight (g/mol) | 185.16 |
| ChEBI | CHEBI:40957 |
| MDL Number | MFCD00004295 |
| SMILES | [Na+].OCCN(CCO)CC([O-])=O |
| IUPAC Name | sodium 2-[bis(2-hydroxyethyl)amino]acetate |
| InChI Key | MFBDBXAVPLFMNJ-UHFFFAOYSA-M |
| Molecular Formula | C6H12NNaO4 |
MilliporeSigma™ HEPES, Sodium Salt, OmniPur™, Calbiochem™,
CAS: 75277-39-3 Molecular Formula: C8H17N2NaO4S Molecular Weight (g/mol): 260.28 MDL Number: MFCD00036463 InChI Key: RDZTWEVXRGYCFV-UHFFFAOYSA-M Synonym: N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid, Sodium Salt PubChem CID: 2724248 ChEBI: CHEBI:46758 IUPAC Name: sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonate SMILES: [Na+].OCCN1CCN(CCS([O-])(=O)=O)CC1
| PubChem CID | 2724248 |
|---|---|
| CAS | 75277-39-3 |
| Molecular Weight (g/mol) | 260.28 |
| ChEBI | CHEBI:46758 |
| MDL Number | MFCD00036463 |
| SMILES | [Na+].OCCN1CCN(CCS([O-])(=O)=O)CC1 |
| Synonym | N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid, Sodium Salt |
| IUPAC Name | sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonate |
| InChI Key | RDZTWEVXRGYCFV-UHFFFAOYSA-M |
| Molecular Formula | C8H17N2NaO4S |
PIPES, 0.2M buffer soln., pH 6.5, low endotoxin, Thermo Scientific™
CAS: 5625-37-6 Molecular Formula: C8H18N2O6S2 Molecular Weight (g/mol): 302.36 MDL Number: MFCD00006159 InChI Key: IHPYMWDTONKSCO-UHFFFAOYSA-N PubChem CID: 79723 ChEBI: CHEBI:44933 IUPAC Name: 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
| PubChem CID | 79723 |
|---|---|
| CAS | 5625-37-6 |
| Molecular Weight (g/mol) | 302.36 |
| ChEBI | CHEBI:44933 |
| MDL Number | MFCD00006159 |
| SMILES | C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O |
| IUPAC Name | 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid |
| InChI Key | IHPYMWDTONKSCO-UHFFFAOYSA-N |
| Molecular Formula | C8H18N2O6S2 |
| Chemical Name or Material | Tris-Tricine Running Buffer Kit |
|---|
Lonza™ BioWhittaker™ Phosphate Buffered Solution (1X)
Serves as a wash solution in a wide variety of laboratory applications. BioWhittaker™ Phosphate Buffered Solution (1X) Phosphate Buffered Saline is manufactured in accordance with cGMP regulations.
| Includes | No Calcium, No Magnesium |
|---|---|
| pH | 7.3 to 7.5 |
| Chemical Name or Material | Phosphate Buffered Saline (PBS) |
| Recommended Storage | 15° to 30°C |
| Sterility | Sterile |
Fisher Science Education™ Tris Borate EDTA
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
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Science Education
A science education product.
Small and/or specialty supplier based on Federal laws and SBA requirements.
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A science education product.
Cytiva HBS-N Buffer, 6x200mL
Degassed and ready to use 0.01 M HEPES pH 7.4, 0.15 M NaCl. For use in Biacore C, and Biacore 3000 SPR systems
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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*STATLAB PHOSPHATE BUFFER 0.2M PH 7.4
NC2183000 PHOSPHATE BUFFER 0.2M PH 7.4
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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